mouse anti glial fibrillary acidic protein gfap (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc mouse anti glial fibrillary acidic protein gfap
Mouse Anti Glial Fibrillary Acidic Protein Gfap, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfap/EAAT2+Rabbit+mAb/pm42863728-141-35-42
Average 94 stars, based on 26 article reviews

mouse anti glial fibrillary acidic protein gfap - by Bioz Stars, 2026-11

94/100 stars

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Incubation:

Article Title: GABA-Induced Exosomes Improve Memory Impairment in Aged Mice
Article Snippet: After washing with 0.1% Tween 20/TBS, the tissues were blocked with Blocking One Histo (Nacalai Tesque) for 1 h at 25 °C. .. Brain sections were incubated with primary antibodies for anti-Iba1 (#17197, Cell Signaling Technology, Danvers, MA, USA), anti-GFAP (#80788, Cell Signaling Technology), anti-p21 (#2974, Cell Signaling Technology), and anti-β Galactosidase (15518-1-AP, Proteintech, Rosemont, IL, USA), and subsequently with secondary antibodies (Alexa Fluor 555 anti-rabbit IgG, Thermo Fischer Scientific) [ ]. .. After staining with Vectashield mounting medium (Vector Laboratories, Burlingame, CA, USA), tissue samples were observed under a fluorescence microscope (EVOS M5000 Cell Imaging System, Thermo Fischer Scientific) under identical exposure settings across all group [ ].

Article Title: GABA-Induced Exosomes Improve Memory Impairment in Aged Mice.
Article Snippet: After washing with 0.1% Tween 20/TBS, the tissues were blocked with Blocking One Histo (Nacalai Tesque) for 1 h at 25 ◦C. .. Brain sections were incubated with primary antibodies for anti-Iba1 (#17197, Cell Signaling Technology, Danvers, MA, USA), anti-GFAP (#80788, Cell Signaling Technology), anti-p21 (#2974, Cell Signaling Technology), and anti-β Galactosidase (15518-1-AP, Proteintech, Rosemont, IL, USA), and subsequently with secondary antibodies (Alexa Fluor 555 anti-rabbit IgG, Thermo Fischer Scientific) [42]. .. After staining with Vectashield mounting medium (Vector Laboratories, Burlingame, CA, USA), tissue samples were observed under a fluorescence microscope (EVOS M5000 Cell Imaging System, Thermo Fischer Scientific) under identical exposure settings across all group [18].

Article Title: B4Galnt1 Deficiency Reverses Severe Neurological Symptoms in a Mouse Model of Tay-Sachs Disease.
Article Snippet: .. Primary antibodies— anti-GM2 (1:200; KM966), anti-GFAP (1:200; 12389, Cell Signaling Technology, USA), anti-Moma2 (1:50; ab33451, Abcam, USA), and anti-NeuN (1:50; 24307, Cell Signaling Technology, USA)—were diluted in blocking solution and incubated overnight at + 4 °C in a humidified chamber. .. Secondary antibodies conjugated with Alexa Fluor dyes were then applied for 1 h at room temperature: goat anti-human DyLight 488 (Thermo, USA) for anti-GM2, goat anti-rabbit Alexa Fluor 488 (Abcam, USA) for anti-GFAP, goat anti-rat Alexa Fluor 568 (Abcam, USA) for anti-Moma2, and goat anti-rabbit Alexa Fluor 568 (Abcam, USA) for anti-NeuN.

Article Title: β-Adducin Restrains PLA2G4A-Dependent Lysosomal Membrane Permeabilization and Neuronal Death in Ischemic Stroke.
Article Snippet: .. Subsequently, they were incubated overnight t 4◦C with the following primary antibodies: anti- β-adducin 1: 200, Origene, #AP20545PU-N), anti-Iba1 (1: 500, Abcam, ab5076), anti-GFAP (1: 500, CST, #3670), anti-Olig2 (1: 500, &D Systems, #AF2418), anti-S100 β (1: 500, Abcam, #ab52642), nti-PLA2G4A (1: 200, CST, #2832), anti-LAMP1 (1: 400, CST, 99437), anti-CTSB (1: 100, Santa Cruz, #sc-365558) and antiTSD (1: 100, Santa Cruz, #sc-377299). ..

Article Title: B4Galnt1 Deficiency Reverses Severe Neurological Symptoms in a Mouse Model of Tay-Sachs Disease
Article Snippet: .. Primary antibodies—anti-GM2 (1:200; KM966), anti-GFAP (1:200; 12389, Cell Signaling Technology, USA), anti-Moma2 (1:50; ab33451, Abcam, USA), and anti-NeuN (1:50; 24307, Cell Signaling Technology, USA)—were diluted in blocking solution and incubated overnight at + 4 °C in a humidified chamber. .. Secondary antibodies conjugated with Alexa Fluor dyes were then applied for 1 h at room temperature: goat anti-human DyLight 488 (Thermo, USA) for anti-GM2, goat anti-rabbit Alexa Fluor 488 (Abcam, USA) for anti-GFAP, goat anti-rat Alexa Fluor 568 (Abcam, USA) for anti-Moma2, and goat anti-rabbit Alexa Fluor 568 (Abcam, USA) for anti-NeuN.

Article Title: An acyclic nucleic acid-modified siRNA targeting CAG expansions for polyglutamine disease treatment
Article Snippet: We separated equal amounts of the protein on 5%–20% or 15% SDS-PAGE gels (Wako, Osaka, Japan) and transferred them to Hybond-P membranes (GE Healthcare, Piscataway, NJ). .. The membranes were incubated with the following primary antibodies: anti-AR (1:2,000; D6F11, Cell Signaling Technology, Beverly, MA), anti-GAPDH (1:5,000; Loading control, Abcam, Cambridge, MA), anti-GFAP (1:1,000; GA5, Cell Signaling Technology, Beverly, MA), anti-ATXN3 (1:5,000; EPR22418-147, Abcam, Cambridge, MA), anti-HTT (1:1,000; D7F7, Cell Signaling Technology, Beverly, MA), anti-TBP (1:1,000; Cell Signaling Technology, Beverly, MA), and anti-IBA-1/AIF-1 (1:1,000; E4O4W, Cell Signaling Technology). ..

Article Title: An acyclic nucleic acid-modified siRNA targeting CAG expansions for polyglutamine disease treatment
Article Snippet: .. The sections were then incubated overnight with the following primary antibodies: anti-polyQ 1C2 (1:20,000; MAB1574, Millipore, Billerica, MA), anti-ChAT (1:1,000, AB144P, Millipore), anti-ATXN3 (1:1,000, MAB5360, Sigma-Aldrich), anti-NeuN (1:100, ABN78A4A, Sigma-Aldrich), anti-GFAP (1:1,000, GA5, Cell Signaling Technology), anti-IBA-1 (1:1,000, 013-27691, Wako), anti-MAP2 (1:2,000, ab5392, Abcam), and anti-Cy5/C3 (1:1,000, ab52060, Abcam). .. For immunohistochemical analysis, the sections were incubated with a secondary antibody conjugated to a polymer as part of the Envision+ system, which contains horseradish peroxidase (Dako Cytomation, Glostrup, Denmark).

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Article Title: Chloride intracellular channel 4 contributes to Aβ-induced cognitive impairment in mice through the regulation of mitochondrial fission. CLIC4 promotes mitochondrial fission.
Article Snippet: Alzheimer’s disease (AD) is a prevalent neurodegenerative disorder characterized by impaired memory and cognitive decline.. The early stages of AD in mice present with neuropathy in the hippocampus.. Excessive mitochondrial fragmentation and dysfunction are critical pathological features of AD.

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